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008			110705s2011 flua sbf 001 0 eng d
020			_a9781439848326 (ebook : PDF)
040			_aFlBoTFG _cFlBoTFG
090			_aQH442 _b.M475 2011
092			_a660.65078 _bW959
100	1		_aWu, William.
245	1	0	_aGene biotechnology _h[electronic resource] / _cWilliam Wu ... [et al.].
250			_a3rd ed.
260			_aBoca Raton : _bCRC Press, _c2011.
300			_axxx, 545 p. : _bill.
500			_a"A CRC title."
504			_aIncludes bibliographical references and index.
505	0		_ach. 1. Strategies for novel research projects and/or research grant funding -- ch. 2. Rapid isolation of specific cDNAs or genes by PCR -- ch. 3. Construction and screening of subtracted and complete expression cDNA libraries -- ch. 4. Subcloning of genes or DNA fragments -- ch. 5. Nonisotopic and isotopic DNA or RNA sequencing -- ch. 6. Bioinformation superhighway and computer databases of nucleic acids and proteins -- ch. 7. Characterization of DNA or genes by southern blot hybridization -- ch. 8. Gene overexpression by sense RNA in mammalian systems -- ch. 9. Gene underexpression in cultured cells and animals by antisense DNA and RNA strategies -- ch. 10. Analysis of gene expression at functional genomic level using northern blotting or PCR -- ch. 11. Analysis of gene expression at proteomic level via western blotting -- ch. 12. Analysis of cellular DNA or abundance of mRNA by radioactivity in Situ hybridization -- ch. 13. Localization of DNA and abundance of mRNA by fluorescence in Situ hybridization -- ch. 14. In Situ PCR hybridization of low copy genes and in Situ RT-PCR detection of low abundance mRNAs -- ch. 15. Isolation and characterization of genes from genomic DNA libraries -- ch. 16. Mouse stem cells as a model mammalian cell line for gene expression -- ch. 17. Strategies for gene double knockout -- ch. 18. Large-scale expression and purification of recombinant proteins in cultured cells -- ch. 19. Quantitative analysis of functional genome by real-time RT-PCR -- ch. 20. High-throughput analysis of gene expression by cutting-edge technology : DNA microarrays (gene chips) -- ch. 21. Construction and screening of human antibody libraries using phage display technology -- ch. 22. Down-regulation of gene expression in mammalian systems via siRNA technology -- ch. 23. Strategies for gene cloning, expression, and identification of protein-protein interaction -- ch. 24. Conditional gene knockout -- ch. 25. How to write a research manuscript for publication in an English journal -- ch. 26. How to protect your discovery and invention : patent 101 -- ch. 27. Determination of transgene copy numbers and practical biocalculation.
520			_a"The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters"--Provided by publisher.
530			_aAlso available in print edition.
538			_aMode of access: World Wide Web.
650		0	_aGenetic engineering _vLaboratory manuals.
650		0	_aMolecular biology _vLaboratory manuals.
655		7	_aElectronic books. _2lcsh
730	0		_iRevision of (work) _aGene biotechnology.
787	1		_iRevision of (work) _tGene biotechnology. _b2nd ed. _dBoca Raton, FL : CRC Press, c2004 _z0849312884 _w(DLC) 2003053183 _w(OCoLC)52288795
776	1		_z9781439848302
856	4	0	_uhttp://marc.crcnetbase.com/isbn/9781439848326 _qapplication/PDF _zDistributed by publisher. Purchase or institutional license may be required for access.
999			_c15854 _d15854