000			03204nam a2200349Ia 4500
001			CRC000E7227PDF
003			FlBoTFG
005			20171224123512.0
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007			cr||||
008			080408s2008 flu sb 001 0 eng d
020			_a9781420006735 (ebook : PDF)
040			_aFlBoTFG _cFlBoTFG
090			_aQH324.9.C7 _bH36 2008
092			_a570.2825 _bH236
245	0	0	_aHandbook of cryo-preparation methods for electron microscopy _h[electronic resource] / _ceditor, Annie Cavalier, Daniele Spehner, and Bruno M. Humbel.
260			_aBoca Raton : _bCRC Press, _c2008.
300			_a704 p.
490	0		_aMethods in visualization
504			_aIncludes bibliographical references and index.
505	0		_aSlam-freezing, metal-mirror freezing / Dani�ele Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing leica empact / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai, and Patrick Schultz -- 2-D crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chr�etien -- CEMOVIS, cryo-electron microscopy of vitreous sections / Jacques Dubochet ... [et al.] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof ... [et al.] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. M�esini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Dani�ele Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa ... [et al.] -- Immunolabeling of ultra-thin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J. C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth
530			_aAlso available in print edition. _bCRC Press (http://www.crcpre
538			_aMode of access: World Wi
650			_aCryobiology _xMetho
650			_aElectron microscopy _xMetho
655			_aElectronic books
700			_2lcsh1 _aCavalier,
700			_aSpehner, D
700			_aHumbel, B
776			_z978084
856			_uhttp://marc.crcnetbase.com/isbn/9781420006735 _qapplication/PDF _zDistributed by publisher. Purchase or institutional license may be required for
999			_c15126 _d15126