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Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / Reiner Westermeier.

By: Westermeier, Reiner [author.].
Material type: materialTypeLabelBookPublisher: Weinheim, Germany : Wiley-VCH, 2016Edition: Fifth edition.Description: 1 online resource.Content type: text Media type: computer Carrier type: online resourceISBN: 9783527695188; 3527695184; 9783527695164; 3527695168; 9783527695195; 3527695192; 9783527695171; 3527695176.Subject(s): Electrophoresis -- Laboratory manuals | DNA -- Separation -- Laboratory manuals | Proteins -- Separation -- Laboratory manuals | SCIENCE / Chemistry / Physical & Theoretical | DNA -- Separation | Electrophoresis | Proteins -- SeparationGenre/Form: Electronic books. | Laboratory manuals.DDC classification: 541/.372 Online resources: Wiley Online Library
Contents:
Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting.
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Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting.

Includes bibliographical references and index.

Online resource; title from PDF title page (John Wiley, viewed March 7, 2016).

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Khulna University of Engineering & Technology
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