"A CRC title."

Includes bibliographical references and index.

ch. 1. Strategies for novel research projects and/or research grant funding -- ch. 2. Rapid isolation of specific cDNAs or genes by PCR -- ch. 3. Construction and screening of subtracted and complete expression cDNA libraries -- ch. 4. Subcloning of genes or DNA fragments -- ch. 5. Nonisotopic and isotopic DNA or RNA sequencing -- ch. 6. Bioinformation superhighway and computer databases of nucleic acids and proteins -- ch. 7. Characterization of DNA or genes by southern blot hybridization -- ch. 8. Gene overexpression by sense RNA in mammalian systems -- ch. 9. Gene underexpression in cultured cells and animals by antisense DNA and RNA strategies -- ch. 10. Analysis of gene expression at functional genomic level using northern blotting or PCR -- ch. 11. Analysis of gene expression at proteomic level via western blotting -- ch. 12. Analysis of cellular DNA or abundance of mRNA by radioactivity in Situ hybridization -- ch. 13. Localization of DNA and abundance of mRNA by fluorescence in Situ hybridization -- ch. 14. In Situ PCR hybridization of low copy genes and in Situ RT-PCR detection of low abundance mRNAs -- ch. 15. Isolation and characterization of genes from genomic DNA libraries -- ch. 16. Mouse stem cells as a model mammalian cell line for gene expression -- ch. 17. Strategies for gene double knockout -- ch. 18. Large-scale expression and purification of recombinant proteins in cultured cells -- ch. 19. Quantitative analysis of functional genome by real-time RT-PCR -- ch. 20. High-throughput analysis of gene expression by cutting-edge technology : DNA microarrays (gene chips) -- ch. 21. Construction and screening of human antibody libraries using phage display technology -- ch. 22. Down-regulation of gene expression in mammalian systems via siRNA technology -- ch. 23. Strategies for gene cloning, expression, and identification of protein-protein interaction -- ch. 24. Conditional gene knockout -- ch. 25. How to write a research manuscript for publication in an English journal -- ch. 26. How to protect your discovery and invention : patent 101 -- ch. 27. Determination of transgene copy numbers and practical biocalculation.

"The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters"--Provided by publisher.

Also available in print edition.

Mode of access: World Wide Web.