Gene biotechnology (Record no. 15854)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 04139nam a2200373Ia 4500 |
| 001 - CONTROL NUMBER | |
| control field | CRC0KE12204PDF |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | FlBoTFG |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20171224123836.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS--GENERAL INFORMATION | |
| fixed length control field | m|||||o||d|||||||| |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr|||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110705s2011 flua sbf 001 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781439848326 (ebook : PDF) |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | FlBoTFG |
| Transcribing agency | FlBoTFG |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (OCLC) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | QH442 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | .M475 2011 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 660.65078 |
| Item number | W959 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wu, William. |
| 245 10 - TITLE STATEMENT | |
| Title | Gene biotechnology |
| Medium | [electronic resource] / |
| Statement of responsibility, etc | William Wu ... [et al.]. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 3rd ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Boca Raton : |
| Name of publisher, distributor, etc | CRC Press, |
| Date of publication, distribution, etc | 2011. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xxx, 545 p. : |
| Other physical details | ill. |
| 500 ## - GENERAL NOTE | |
| General note | "A CRC title." |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | ch. 1. Strategies for novel research projects and/or research grant funding -- ch. 2. Rapid isolation of specific cDNAs or genes by PCR -- ch. 3. Construction and screening of subtracted and complete expression cDNA libraries -- ch. 4. Subcloning of genes or DNA fragments -- ch. 5. Nonisotopic and isotopic DNA or RNA sequencing -- ch. 6. Bioinformation superhighway and computer databases of nucleic acids and proteins -- ch. 7. Characterization of DNA or genes by southern blot hybridization -- ch. 8. Gene overexpression by sense RNA in mammalian systems -- ch. 9. Gene underexpression in cultured cells and animals by antisense DNA and RNA strategies -- ch. 10. Analysis of gene expression at functional genomic level using northern blotting or PCR -- ch. 11. Analysis of gene expression at proteomic level via western blotting -- ch. 12. Analysis of cellular DNA or abundance of mRNA by radioactivity in Situ hybridization -- ch. 13. Localization of DNA and abundance of mRNA by fluorescence in Situ hybridization -- ch. 14. In Situ PCR hybridization of low copy genes and in Situ RT-PCR detection of low abundance mRNAs -- ch. 15. Isolation and characterization of genes from genomic DNA libraries -- ch. 16. Mouse stem cells as a model mammalian cell line for gene expression -- ch. 17. Strategies for gene double knockout -- ch. 18. Large-scale expression and purification of recombinant proteins in cultured cells -- ch. 19. Quantitative analysis of functional genome by real-time RT-PCR -- ch. 20. High-throughput analysis of gene expression by cutting-edge technology : DNA microarrays (gene chips) -- ch. 21. Construction and screening of human antibody libraries using phage display technology -- ch. 22. Down-regulation of gene expression in mammalian systems via siRNA technology -- ch. 23. Strategies for gene cloning, expression, and identification of protein-protein interaction -- ch. 24. Conditional gene knockout -- ch. 25. How to write a research manuscript for publication in an English journal -- ch. 26. How to protect your discovery and invention : patent 101 -- ch. 27. Determination of transgene copy numbers and practical biocalculation. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | "The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters"--Provided by publisher. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Also available in print edition. |
| 538 ## - SYSTEM DETAILS NOTE | |
| System details note | Mode of access: World Wide Web. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genetic engineering |
| Form subdivision | Laboratory manuals. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular biology |
| Form subdivision | Laboratory manuals. |
| 655 #7 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| Source of term | lcsh |
| 730 0# - ADDED ENTRY--UNIFORM TITLE | |
| -- | Revision of (work) |
| Uniform title | Gene biotechnology. |
| 787 1# - NONSPECIFIC RELATIONSHIP ENTRY | |
| Display text | Revision of (work) |
| Title | Gene biotechnology. |
| Edition | 2nd ed. |
| Place, publisher, and date of publication | Boca Raton, FL : CRC Press, c2004 |
| International Standard Book Number | 0849312884 |
| Record control number | (DLC) 2003053183 |
| -- | (OCoLC)52288795 |
| 776 1# - ADDITIONAL PHYSICAL FORM ENTRY | |
| International Standard Book Number | 9781439848302 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://marc.crcnetbase.com/isbn/9781439848326 |
| Electronic format type | application/PDF |
| Public note | Distributed by publisher. Purchase or institutional license may be required for access. |
No items available.