Westermeier, Reiner,
Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / Reiner Westermeier. - Fifth edition. - 1 online resource.
Includes bibliographical references and index.
Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting.
9783527695188 3527695184 9783527695164 3527695168 9783527695195 3527695192 9783527695171 3527695176
10.1002/9783527695188 doi
Electrophoresis--Laboratory manuals.
DNA--Separation--Laboratory manuals.
Proteins--Separation--Laboratory manuals.
SCIENCE / Chemistry / Physical & Theoretical
DNA--Separation.
Electrophoresis.
Proteins--Separation.
Electronic books.
Laboratory manuals.
QP519.9.E434
541/.372
Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / Reiner Westermeier. - Fifth edition. - 1 online resource.
Includes bibliographical references and index.
Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting.
9783527695188 3527695184 9783527695164 3527695168 9783527695195 3527695192 9783527695171 3527695176
10.1002/9783527695188 doi
Electrophoresis--Laboratory manuals.
DNA--Separation--Laboratory manuals.
Proteins--Separation--Laboratory manuals.
SCIENCE / Chemistry / Physical & Theoretical
DNA--Separation.
Electrophoresis.
Proteins--Separation.
Electronic books.
Laboratory manuals.
QP519.9.E434
541/.372